My dac Nagra tube dac upsampling everytthing

Since Audionirvana also can uppsampel I Wonder if this double upsampling has a negativ effekt on SQ
Should I use A. with no upsampling?

The only way to tell is to try it.

According to Nagra specs it upsample everything to DSD256, that said you could try to compare
a) Audirvana no upsampling —> Nagra
b) Audirvana upsampling to DSD256 —> Nagra
in the b) modality you should skip internal Nagra upsampling.
In his way you could decide which modality is best for you.
Of course you can try all the intermediate Audirvana upsampling modalities too
… your ears will give you the solution …